Single Cell Biophysics. Leader : Gilles Charvin
Single cells time-lapse microscopy has proven very powerful to investigate the dynamics of regulatory networks in a quantitative manner. As opposed to bulk experiments, single cell techniques give the possibility to measure the whole variety of behaviors of a given biological system. However, tracking cells over time is intrinsically limited to several generations, due to cell exponential growth (see Figure). As a result, a large class of biological issues spanning larger timescales (typically 10-100 cell generations), e.g. epigenetic processes, can’t be followed in real-time. Our goal is to develop novel biophysical techniques to expand the range of tools available in single cell biology, with a main focus on the study of replicative aging in yeast. Other applications include the monitoring of the regulation of chromatin silencing, the propagation of prion, and the adaptation of cell physiology in a changing environment.
Legend : Synchronization of yeast cells by periodic pulsing of CDC20 in a cdc20- strain. TUB1-GFP is used to mark the microtubules spindle and the CDC10-YFP septin fusion to score budding and division. Taken from G. Charvin et al., PLoS ONE (2008).