Aller au contenu. | Aller à la navigation

Outils personnels

Navigation
Vous êtes ici : Accueil / Équipes / Génétique des Variations Intra-Espèce - G. Yvert / Publications / A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch.

A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch.

Hélène Duplus-Bottin, Martin Spichty, Gérard Triqueneaux, Christophe Place, Philippe E Mangeot, Théophile Ohlmann, Franck Vittoz, and Gaël Yvert (2021)

Elife, 10.

Optogenetics enables genome manipulations with high spatiotemporal resolution,opening exciting possibilities for fundamental and applied biological research.Here, we report the development of LiCre, a novel light-inducible Cre recombinase.LiCre is made of a single flavin-containing protein comprising the AsLOV2photoreceptor domain of Avena sativa fused to a Cre variant carrying destabilizingmutations in its N-terminal and C-terminal domains. LiCre can be activated withinminutes of illumination with blue light, without the need of additional chemicals.When compared to existing photoactivatable Cre recombinases based on two splitunits, LiCre displayed faster and stronger activation by light as well as a lowerresidual activity in the dark. LiCre was efficient both in yeast, where it allowedus to control the production of β-carotene with light, and in human cells. Given itssimplicity and performances, LiCre is particularly suited for fundamental andbiomedical research, as well as for controlling industrial bioprocesses.

 
automatic medline import

Actions sur le document