Aller au contenu. | Aller à la navigation

Outils personnels

Vous êtes ici : Accueil / Équipes / Génétique des Variations Intra-Espèce - G. Yvert / Publications / Natural single-nucleosome epi-polymorphisms in yeast.

Natural single-nucleosome epi-polymorphisms in yeast.

Muniyandi Nagarajan, Jean-Baptiste Veyrieras, Maud de Dieuleveult, Helene Bottin, Steffen Fehrmann, Anne-Laure Abraham, Severine Croze, Lars M Steinmetz, Xavier Gidrol, and Gael Yvert (2010)

PLoS Genet, 6(4):e1000913.

Epigenomes commonly refer to the sequence of presence/absence of specific epigenetic marks along eukaryotic chromatin. Complete histone-borne epigenomes have now been described at single-nucleosome resolution from various organisms, tissues, developmental stages, or diseases, yet their intra-species natural variation has never been investigated. We describe here that the epigenomic sequence of histone H3 acetylation at Lysine 14 (H3K14ac) differs greatly between two unrelated strains of the yeast Saccharomyces cerevisiae. Using single-nucleosome chromatin immunoprecipitation and mapping, we interrogated 58,694 nucleosomes and found that 5,442 of them differed in their level of H3K14acetylation, at a false discovery rate (FDR) of 0.0001. These Single Nucleosome Epi-Polymorphisms (SNEPs) were enriched at regulatory sites and conserved non-coding DNA sequences. Surprisingly, higher acetylation in one strain did notimply higher expression of the relevant gene. However, SNEPs were enriched in genes of high transcriptional variability and one SNEP was associated with the strength of gene activation upon stimulation. Our observations suggest a high level of inter-individual epigenomic variation in natural populations, with essential questions on the origin of this diversity and its relevance to gene x environment interactions.

automatic medline import

Actions sur le document