NIR-to-NIR Two-Photon Scanning Laser Microscopy Imaging of Single Nanoparticles Doped by Yb-III Complexes
This article describes the potentialities of ytterbium-based (nano)probes for microscopy imaging in the challenging NIR-to-NIR configuration. These probes combined the intrinsic advantages of two-photon excitation microscopy (confocal character, 3D resolution, NIR excitation) with those of lanthanide emission (sharp band located at 1000 nm for Yb(III), large Stokes’ shift). New probes featuring increased stability, even in water, are described by embedding the [NBu4]3[YbL3] complex in a dispersible organosilica matrix, leading to the formation of stable and bright nanoemitters. Finally, the detection limit are pushed down to single nano-objects using two-photon scanning microscopy in the NIR-to-NIR configuration, thanks to the high brightness of {[NBu4]3[YbL3]:NPs}. These NIR nanoemitters combining high brightness and enhanced stability in buffers should offer interesting potentialities for thick-tissue imaging using NIR-to-NIR biphotonic microscopy. |
Bourdolle, A ; D'Aleo, A ; Philippot, C ; Baldeck, PL ; Guyot, Y ; Dubois, F ; Ibanez, A ; Andraud, C ; Brasselet, S ; Maury, O
CHEMPHYSCHEM Volume: 17, Issue: 1, Pages: 128-135, DOI: 10.1002/cphc.201500814, Published: JAN 4 2016