Inorganic cellular chemistry studies metal complexes in a biological context. In this talk, we will present two research axes.

(1) Oxidative burst is a relevant biomedical target because it is involved in a wide range of diseases. The beneficial outcome of superoxide removal is now well documented.^{1, 2} SOD-mimics³ are small complexes that reproduce the activity of superoxide dismutases, natural proteins that catalytically dismutate the superoxide anion. Activated macrophages, which produce ROS and RNS fluxes, constitute a relevant model to challenge antioxidant activity in a cellular context and they were used to evaluate the intracelllular anti-ROS and RNS activities of a Mn-complex.⁵

 (2) We have shown that metal-CO derivatives can be used as IR-probes for sub-cellular imaging, using a cutting-edge technique coupling and AFM with and IR laser —AFMIR—⁶ or synchrotron based FTIR.⁷ Interestingly enough, metal-CO derivatives can also be designed as fluorescent, leading to bimodal probes based on a single molecular core enabling bimodal imaging, both at the sub-cellular level and tissue level, and we have coined the term SCoMPI for these Single Core Multimodal Probe for Imaging^8,9,10 that we will present in the talk. In addition, one of the interests of IR is its easy implementation for direct quantification.¹¹ These SCoMPIs are thus very promising to tag small molecules for imaging and quantification in biological media.

REFERENCES :

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2.D. Salvemini, C. Muscoli, D. P. Riley and S. Cuzzocrea, Pulmonary Pharmacology and Therapeutics, 2002, 15, 439

3. O. Iranzo, Bioorganic Chemistry, 2011, 39, 73

4. F. Cisnetti, A. S. Lefevre, R. Guillot, F. Lambert, G. Blain, E. Anxolabéhère-Mallart and C. Policar, Eur. J. Inorg. Chem., 2007, 4472

5.A.-S. Bernard, C. Giroud, H. Y. V. Ching, A. Meunier, V. Ambike, C. Amatore, M. Guille Collignon, F. Lemaître and C. Policar, Dalton Trans., 2012, 41, 6399

6. C. Policar, J. B. Waern, M. A. Plamont, S. Clède, C. Mayet, R. Prazeres, J.-M. Ortega, A. Vessières and A. Dazzi, Angew. Chem. Int. Ed., 2011, 860

7. S. Clède, F. Lambert, C. Sandt, Z. Gueroui, N. Delsuc, P. Dumas, A. Vessières and C. Policar, Biotechnology Advances, 2013, 31, 393

8.S. Clède, F. Lambert, C. Sandt, Z. Gueroui, M.-A. Plamont, P. Dumas, A. Vessières and C. Policar, Chem. Commun., 2012, 48, 7729

9. S. Clède, C. Policar, Chem. Eur. J. 2015, 21, 942 – 958

10. S. Clède, N. Delsuc, C. Laugel, F. Lambert, C. Sandt, A. Baillet-Guffroy, C. Policar, Chem. Commun. 51, 2015, 2687-2689

11. S. Clède, F. Lambert, R. Saint-Fort, M.-A. Plamont, H. Bertrand, A. Vessières, C. Policar, Chem. Eur. J., 2014, 8714-822

contact: clotilde.policar @ ens.fr