Annuaire
Pr. Kazuya KIKUCHI
| Quand ? |
21/11/2016 à 17:00 |
|---|---|
| Où ? |
Schrödinger lecture room Monod |
| Contact |
Jens Hasserodt |
Development of fluorescent probes for Memory imaging of membrane protein localization
One of the great challenges in the post-genome era is to clarify the biological significance of intracellular molecules directly in living cells. If we can visualize a molecule in action, it is possible to acquire biological information, which is unavailable if we deal with cell homogenates. One possible approach is to design and synthesize chemical probes that can convert biological information to chemical output. In this talk, molecular design strategies for fluorescence imaging probes are introduced.
Fluorescence protein labeling by synthetic probes is a powerful approach to investigate protein function and localization inside living cells. Its potential advantage is that various fluorescent molecules are available as labeling reagents, and the timing of protein labeling is easily controlled. Because of these characteristics, this method is attracting attention as an alternative of fluorescent proteins. In the reported labeling system, washing procedures are required to remove the free probes from cells. However, if the probes are not completely washed out, the remaining probes cause the reduction of the signal-to-noise ratio. For the solution of this limitation photoactive yellow protein (PYP) tag will be introduced.