Annuaire
Jérémy LEBRETON
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Le 17/06/2022, de 11:00 à 12:00 |
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| S'adresser à | Equipe Bernard - Salle des Thèses |
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RNAs and transcription are emerging as important factors that controls chromosome organization and dynamics. If the idea of a rigid and stable RNA scaffold has now been abandoned, several lines of evidence suggest that RNA form a dynamic mesh that controls chromatin structure. ncRNAs associate with chromatin and recruit architectural proteins, transcription has been identified as a roadblock for SMC loop extruders and the activity of RNA binding proteins is essential to maintain chromosome structure in interphase and metaphase. In this context we found that the condensin complex physically interacts with RNA binding proteins involved in RNA catabolism and transcription termination in fission yeast. We further found that Rrp6, a catalytic subunit of the nuclear exosome, is important to maintain genome integrity at the rDNA if condensin is impaired. Degradation of Rrp6 in condensin-deficient background led to loss of cell viability and to rDNA missegregation. We additionally identified slight modification of mitotic chromosome organization upon Rrp6 degradation. Our data indicate that the RNA exosome has a small role in the general organization of mitotic chromosomes and that some specific regions, such as the rDNA, requires its presence to maintain their stability during mitosis.