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You are here: Home / Teams / Chromatin Dynamics in Mitotic Chromosome Assembly - P. Bernard / Publications / A genetic screen for functional partners of condensin in fission yeast.

A genetic screen for functional partners of condensin in fission yeast.

Xavier Robellet, Lydia Fauque, Penelope Legros, Esther Mollereau, Stephane Janczarski, Hugues Parrinello, Jean-Pierre Desvignes, Morgane Thevenin, and Pascal Bernard (2014)

G3 (Bethesda), 4(2):373-81.

Mitotic chromosome condensation is a prerequisite for the accurate segregation of chromosomes during cell division, and the conserved condensin complex a central player of this process. However, how condensin binds chromatin and shapes mitotic chromosomes remain poorly understood. Recent genome-wide binding studies showingthat in most species condensin is enriched near highly expressed genes suggest aconserved link between condensin occupancy and high transcription rates. To gaininsight into the mechanisms of condensin binding and mitotic chromosome condensation, we searched for factors that collaborate with condensin through a synthetic lethal genetic screen in the fission yeast Schizosaccharomyces pombe. We isolated novel mutations affecting condensin, as well as mutations in four genes not previously implicated in mitotic chromosome condensation in fission yeast. These mutations cause chromosome segregation defects similar to those provoked by defects in condensation. We also identified a suppressor of the cut3-477 condensin mutation, which largely rescued chromosome segregation duringanaphase. Remarkably, of the five genes identified in this study, four encode transcription co-factors. Our results therefore provide strong additional evidence for a functional connection between chromosome condensation and transcription.

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