Annuaire
Titration of Apparent In-Cellula Affinities of Protein-Protein Interactions.
Chembiochem, 23(4):e202100640.
A genetic assay permits simultaneous quantification of two interacting proteinsand their bound fraction at the single-cell level using flow cytometry. Apparentin-cellula affinities of protein-protein interactions can be extracted from theacquired data through a titration-like analysis. The applicability of thisapproach is demonstrated on a diverse set of interactions with proteins fromdifferent families and organisms and with in-vitro dissociation constants rangingfrom picomolar to micromolar.
automatic medline import
Actions sur le document