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You are here: Home / Teams / Systems Biology of Decision Making - O. Gandrillon / Publications (not up to date) / Serial analysis of gene expression in the silkworm, Bombyx mori.

Serial analysis of gene expression in the silkworm, Bombyx mori.

Jianhua Huang, Xuexia Miao, Weirong Jin, Pierre Couble, Kasuei Mita, Yong Zhang, Wenbin Liu, Leijun Zhuang, Yan Shen, Celine Keime, Olivier Gandrillon, Patrick Brouilly, Jerome Briolay, Guoping Zhao, and Yongping Huang (2005)

Genomics, 86(2):233-41.

The silkworm Bombyx mori is one of the most economically important insects and serves as a model for Lepidoptera insects. We used serial analysis of gene expression (SAGE) to derive profiles of expressed genes during the developmentallife cycle of the silkworm and to create a reference for understanding silkworm metamorphosis. We generated four SAGE libraries, one from each of the four developmental stages of the silkworm. In total we obtained 257,964 SAGE tags, ofwhich 39,485 were unique tags. Sorted by copy number, 14.1% of the unique tags were detected at a median to high level (five or more copies), 24.2% at lower levels (two to four copies), and 61.7% as single copies. Using a basic local alignment search tool on the EST database, 35% of the tags matched known silkworm expressed sequence tags. SAGE demonstrated that a number of the genes were up- or down-regulated during the four developmental phases of the egg, larva, pupa, andadult. Furthermore, we found that the generation of longer cDNA fragments from SAGE tags constituted the most efficient method of gene identification, which facilitated the analysis of a large number of unknown genes.

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