Lumen charge governs memristive ion transport in β-barrel nanopores
| When |
Nov 18, 2024
from 11:00 to 12:00 |
|---|---|
| Where | Salle des thèses |
| Contact Name | Fabien Montel |
| Attendees |
Aleksandra Radenovic |
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β-barrel nanopores are involved in crucial biological processes, from ATP export in mitochondria to bacterial resistance, and represent a promising platform for emerging sequencing technologies. However, the fundamental principles governing ion transport across these nanopores remain poorly understood due to their extreme confinement and heterogeneous physico-chemical properties. In this study, we integrate experimental, numerical, and theoretical approaches to elucidate ion transport mechanisms in these biological nanopores. We identify and characterise two distinct nonlinear phenomena: open-pore rectification and gating. Through extensive mutation analysis of aerolysin nanopores, we demonstrate that open-pore rectification is caused by ionic accumulation driven by the distribution of lumen charges. Additionally, we provide converging evidence suggesting that gating is controlled by electric fields pulling on lumen charges and controlled by local structural deformations. Our findings pave the way for the design of optimised biosensors as well as highly tunable and compact ionic memristors and diodes for biological computing applications. They also establish a rigorous framework for the characterisation of biological ion transport processes.
List of coworkers: Simon Finn Mayer, Marianna Fanouria Mitsioni, Lukas van den Heuvel, Paul Robin, Nathan Ronceray, Maria Josefina Marcaida, Luciano A. Abriata, Lucien F. Krapp, Jana S. Anton, Sarah Soussou, Justin Jeanneret-Grosjean, Alessandro Fulciniti, Alexia Möller, Sarah Vacle, Lely Feletti, Henry Brinkerhoff, Andrew H. Laszlo, Jens H. Gundlach, Theo Emmerich, Matteo Dal Peraro, Aleksandra Radenovic