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Fluorogenic Probes Responding to enzyme activity
His probes are built from three, sometimes four components and act as substrates for selected target enzymes. Enzyme turnover results in the release of a highly insoluble fluorophore that only fluoresces in the solid state. The usefulness of this approach has already been demonstrated in the tagging of live cells competent in the targeted enzyme activity. Currently, J. Hasserodt’s team seeks to demonstrate its usefulness for high-throughput screening of cell collections via cytometry (FACS). Research on the modification of each of the three components is ongoing so as to adapt the probes to further enzyme activities of interest in life and disease, and to optimize their physicochemical performance (response rate; emitted color; cell penetration efficacy; solubility).