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The catalytic triad of rice NARROW LEAF1 involves H234.

Ling-Yun Huang, Na-Nv Liu, Wei-Fei Chen, Xia Ai, Hai-Hong Li, Ze-Lin Zhang, Xi-Miao Hou, Philippe Fossé, Olivier Mauffret, Dong-Sheng Lei, Stephane Rety, and Xu-Guang Xi (2024)

Nat Plants, 10(5):743-748.

NARROW LEAF1 (NAL1) exerts a multifaceted influence on leaf morphology and cropyield. Recent crystal study proposed that histidine 233 (H233) is part of thecatalytic triad. Here we report that unlike suggested previously, H234 instead ofH233 is a component of the catalytic triad alongside residues D291 and S385 inNAL1. Remarkably, residue 233 unexpectedly plays a pivotal role in regulatingNAL1's proteolytic activity. These findings establish a strong foundation forutilizing NAL1 in breeding programs aimed at improving crop yield.

 
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