Mehdi Doumane, Léia Colin, Alexis Lebecq,

Aurélie Fangain, FD Stevens, Joseph Bareille,

Olivier Hamant, Youssef Belkhadir, T Munnik,

Yvon Jaillais #, Marie-Cécile Caillaud #.

Inducible depletion of PI(4,5)P2 by the

synthetic iDePP system in Arabidopsis.

Nature Plants 7 587-597 (2021)

Open on bioRxiv 2020.05.13.091470;

doi: https://doi.org/10.1101/2020.05.13.091470

The following marker lines have been

deposited to the NASC:

MAP-mCH-dOCRL Col0 background N2110785 (HygroR)

MAP-mCH-dOCRLdead Col0 background N2110786 (HygroR)

MAP-3xmCH Col0 background N2110787 (HygroR)

MAP-mCH-dOCRL x mCit-2xPH(PLC) (P24Y) Col0 background N2110788 (HygroR + BastaR)

MAP-mCH-dOCRLdead x mCit-2xPH(PLC) (P24Y) Col0 background N2110789 (HygroR + BastaR)

MAP-3xmCH x mCit-2xPH(PLC) (P24Y) Col0 background N2110790 (HygroR + BastaR)

MAP-mCH-dOCRL x mCit-TUBBY (P15Y) Col0 background N2110791 (HygroR + BastaR)

MAP-mCH-dOCRLdead x mCit-TUBBY (P15Y) Col0 background N2110792 (HygroR + BastaR)

MAP-3xmCH x mCit-TUBBY (P15Y) Col0 background N2110793 (HygroR + BastaR)

MAP-mCH-dOCRL x mCit-P4M (PI4P marker) Col0 background N2110794 (HygroR + BastaR)

MAP-mCH-dOCRLdead x mCit-P4M (PI4P marker) Col0 background N2110795 (HygroR + BastaR)

MAP-3xmCH x mCit-P4M (PI4P marker) Col0 background N2110796 (HygroR + BastaR)

Sequences:

Codon optimized drosophila OCRL (dOCRL) docrl-codon-optimized-arabidopsis.gb

ENTRY vectors:

UBQ10::GVG-6xUAS in pDONR-P4R-P1 ubq10promgvg-te96xuas-35sminipDONRP4P1R.gb

MAP-mCHERRY in pDONR207 MAP-mCHERRY-pDONR207.gb

dOCRL catalytic domain in pDONR-P2R-P3 dOCRL-168-509_pDONRP2RP3.gb

2xmCHERRY in pDONR-P2RP3 2xmCHERRY_4xMyc_pDONRP2RP3.gb

DESTINATION vectors

MAP-mCH-dOCRL (UBQ10::GVG-6xUAS::MAP-mCHERRY-dOCRL/pH7m34GW)

MAP-3xmCHERRY (UBQ10::GVG-6xUAS::MAP-mCHERRY-2xmCHERRY/pH7m34GW)

Other lines and plasmids will be send upon request