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Accueil du site > Animations Scientifiques > Séminaires 2008 > 4C technology : uncovering the multi-dimensional organisation of the genome
4C technology : uncovering the multi-dimensional organisation of the genome
Orateur :
Wouter De laat, Department of Cell Biology and Genetics, Erasmus MC, Rotterdam
Salle :
118
Sujet :
The architecture of DNA in the cell nucleus is an emerging key contributor to genome function. For example, genes move in the nuclear interior when they get activated. We recently developed 4C technology, a high-throughput technique that combines 3C technology with tailored micro-arrays to uniquely allow for an unbiased genome-wide search for DNA loci that interact in the nuclear space. Data based on 4C technology will be presented that investigate the nuclear environments which genes leave and enter when they get activated. Furthermore, it will be demonstrated that 4C detects deletions, balanced inversions and translocations in patient samples at a resolution ( 7kb) that allows immediate sequencing of the breakpoints. 4C technology therefore also offers a high-resolution genomic approach that can identify both balanced and unbalanced genomic rearrangements.
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