Skip to content. | Skip to navigation

Personal tools

Sections
You are here: Home / Teams / Physical Biology of Chromatin - D. Jost / Publications / Genome-wide mapping of individual replication fork velocities using nanopore sequencing.

Genome-wide mapping of individual replication fork velocities using nanopore sequencing.

Bertrand Theulot, Laurent Lacroix, Jean-Michel Arbona, Gael A Millot, Etienne Jean, Corinne Cruaud, Jade Pellet, Florence Proux, Magali Hennion, Stefan Engelen, Arnaud Lemainque, Benjamin Audit, Olivier Hyrien, and Benoît Le Tallec (2022)

Nat Commun, 13(1):3295.

Little is known about replication fork velocity variations along eukaryoticgenomes, since reference techniques to determine fork speed either provide nosequence information or suffer from low throughput. Here we presentNanoForkSpeed, a nanopore sequencing-based method to map and extract the velocityof individual forks detected as tracks of the thymidine analoguebromodeoxyuridine incorporated during a brief pulse-labelling of asynchronouslygrowing cells. NanoForkSpeed retrieves previous Saccharomyces cerevisiae meanfork speed estimates (≈2 kb/min) in the BT1 strain exhibiting highly efficientbromodeoxyuridine incorporation and wild-type growth, and precisely quantifiesspeed changes in cells with altered replisome progression or exposed tohydroxyurea. The positioning of >125,000 fork velocities provides a genome-widemap of fork progression based on individual fork rates, showing a uniform forkspeed across yeast chromosomes except for a marked slowdown at known pausingsites.

 
automatic medline import

Document Actions