Directory
Protocols
Our protocols to detect D-loop joint molecules and their extension by DNA polymerases in S. cerevisiae upon site-specific DNA break formation have been compiled in a recent JoVE paper, available here. See also our D-loop Capture lab protocol.
Common yeast protocols:
- CRISPR/Cas9-mediated genome editing with single and dual-guide. Based on the protocol by Agier et al. 2021.
- Yeast transformation with LiAc and heatshock, with the magic step!
- Yeast transformation by electroporation
- Colony PCR (works every time)
- MIR translocation assay
- DNA extraction
- Protein extraction
