Protocols and repository
Codes and softwares:
In-house protocols:
- D-loop Capture (DLC) and D-loop Extension (DLE) assays have been compiled in a recent JoVE paper, available here. See also our D-loop Capture lab protocol.
- Chromosome rearrangement Capture (CR-C) assay, described in Reitz et al. 2023. Lab protocol.
- Optimized crosslink protocol and Hi-C lab protocol.
- ssDNA-specific Hi-C (ssHi-C) described in Dumont et al. 2024. Lab protocol and analysis software.
- MIR translocation assay, as described in Piazza et al. 2017. Lab protocol.
Optimized everyday yeast protocols:
- CRISPR/Cas9-mediated genome editing with single and dual-guide. Based on the protocol by Agier et al. 2021.
- Yeast transformation, with the magic step!
- Colony PCR (works every time)