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Our protocols to detect D-loop joint molecules and their extension by DNA polymerases in S. cerevisiae upon site-specific DNA break formation have been compiled in a recent JoVE paper, available here. See also our D-loop Capture lab protocol.

Common yeast protocols:

  • CRISPR/Cas9-mediated genome editing with single and dual-guide. Based on the protocol by Agier et al. 2021.
  • Yeast transformation with LiAc and heatshock, with the magic step!
  • Yeast transformation by electroporation
  • Colony PCR (works every time)
  • MIR translocation assay
  • DNA extraction
  • Protein extraction