Intervenant(s)
Soutenance de thèse de M. Zheng ZHENG du Laboratoire de Chimie sous la direction de Mme Chantal ANDRAUD
Description générale
The thesis mainly includes two aspects as describled below:
I. Design of two-photon absorbing probes for the in-vivo monitoring of oxygen pressure
Four new fluorene-based two-photon absorbing (TPA) chromophores have been synthesized and their one- and two-photon photophysical properties have been investigated. The optimum chromophore was successfully attached covalently to a Pd-porphyrin complex by the click reaction. Two new compounds contain four or eight TPA chromophores donor connected to the Pd-porphyrin acceptor. The result demonstrate that the incorporation of a suitable TPA chromophore can effectively enhance the TPA of the system, allowing efficient sensitivity towards oxygen.
II. Development of new near-infrared chromophores and nanoparticles for two-photon fluorescence bioimaging
A new series of asymmetrical fluorene-based chromophores were designed and synthesized. Their structure-property relationships have been systematically investigated. It was found that most of chromophores exhibit aggregation-induced emission behaviors in the near-infrared (NIR) region. In addition, a micelle/silica coprotection strategy was proposed to prepare nanoparticles with a less polar interior, which can be used to conserve optical properties of dipole chromophores in aqueous solution. The two-photon excited fluorescence (TPEF) measurements indicate that they all display obvious TPA activities in organic solvent and aqueous suspension. Both the NIR-emissive aggregates and nanoparticles have been successfully used for TPEF imaging of blood vessels inside mouse ear skin.
I. Design of two-photon absorbing probes for the in-vivo monitoring of oxygen pressure
Four new fluorene-based two-photon absorbing (TPA) chromophores have been synthesized and their one- and two-photon photophysical properties have been investigated. The optimum chromophore was successfully attached covalently to a Pd-porphyrin complex by the click reaction. Two new compounds contain four or eight TPA chromophores donor connected to the Pd-porphyrin acceptor. The result demonstrate that the incorporation of a suitable TPA chromophore can effectively enhance the TPA of the system, allowing efficient sensitivity towards oxygen.
II. Development of new near-infrared chromophores and nanoparticles for two-photon fluorescence bioimaging
A new series of asymmetrical fluorene-based chromophores were designed and synthesized. Their structure-property relationships have been systematically investigated. It was found that most of chromophores exhibit aggregation-induced emission behaviors in the near-infrared (NIR) region. In addition, a micelle/silica coprotection strategy was proposed to prepare nanoparticles with a less polar interior, which can be used to conserve optical properties of dipole chromophores in aqueous solution. The two-photon excited fluorescence (TPEF) measurements indicate that they all display obvious TPA activities in organic solvent and aqueous suspension. Both the NIR-emissive aggregates and nanoparticles have been successfully used for TPEF imaging of blood vessels inside mouse ear skin.
Complément
Salle des Thèses - Site Monod - ENS de Lyon
Disciplines