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Development of Far-Red / Near-Infrared Luminescent Chromophores and Nanoparticles for in vivo Biphotonic Applications

Soutenance de thèse

Lundi 26 sep 2016
14h00
Soutenance de thèse de M. Zheng ZHENG du Laboratoire de Chimie sous la direction de Mme Chantal ANDRAUD

Intervenant(s)

Soutenance de thèse de M. Zheng ZHENG du Laboratoire de Chimie sous la direction de Mme Chantal ANDRAUD

Description générale
The thesis mainly includes two aspects as describled below:
I. Design of two-photon absorbing probes for the in-vivo monitoring of oxygen pressure
Four new fluorene-based two-photon absorbing (TPA) chromophores have been synthesized and their one- and two-photon photophysical properties have been investigated. The optimum chromophore was successfully attached covalently to a Pd-porphyrin complex by the click reaction. Two new compounds contain four or eight TPA chromophores donor connected to the Pd-porphyrin acceptor. The result demonstrate that the incorporation of a suitable TPA chromophore can effectively enhance the TPA of the system, allowing efficient sensitivity towards oxygen.
II. Development of new near-infrared chromophores and nanoparticles for two-photon fluorescence bioimaging
A new series of asymmetrical fluorene-based chromophores were designed and synthesized. Their structure-property relationships have been systematically investigated. It was found that most of chromophores exhibit aggregation-induced emission behaviors in the near-infrared (NIR) region. In addition, a micelle/silica coprotection strategy was proposed to prepare nanoparticles with a less polar interior, which can be used to conserve optical properties of dipole chromophores in aqueous solution. The two-photon excited fluorescence (TPEF) measurements indicate that they all display obvious TPA activities in organic solvent and aqueous suspension. Both the NIR-emissive aggregates and nanoparticles have been successfully used for TPEF imaging of blood vessels inside mouse ear skin.
Complément

Salle des Thèses - Site Monod - ENS de Lyon

Disciplines