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Peter Meister — Putting things in place : sequences and signals for genome nuclear organization
Speaker :
Peter Meister, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
When :
Wednesday 8 June at 11am
Where :
C023 (RDC LR6 côté Centre Blaise Pascal)
Title :
Putting things in place : sequences and signals for genome nuclear organization
Abstract :
To understand whether the spatial organization of the genome reflects the cell’s differentiated state, we have examined whether genes assume specific subnuclear positions during C. elegans development. Monitoring the radial position of developmentally controlled promoters in embryos and larval tissues, we found that small integrated transgenes bearing three different tissue-specific promoters have no preferential position in nuclei of undifferentiated embryos. However, in differentiated cells they shifted stably towards the nuclear lumen when activated, or to the nuclear envelope when silent. We uncovered two additional parameters which influence subnuclear positioning. First, high copy number repeats targets arrays to the nuclear rim, even in the presence of active housekeeping promoters. Tissue-specific activation of promoters in the arrays overrides perinuclear anchoring. Second, some promoters contain sequences which target them to the nuclear periphery, both in their active and inactive form. Using genetic and biochemical methods, we are now using our system to uncover the molecular machinery involved in subnuclear positioning of genes.
Joint work with Benjamin D. Towbin, Sabine Rohner, Ragna Sack, Veronique Kalck and Susan M. Gasser.
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