Partenaires
Rechercher
Accueil du site > Animations Scientifiques > Séminaires 2013 > Marcus Buschbeck - MacroH2A contributes to the establishment and stabilization of differentiated epigenomes
Marcus Buschbeck - MacroH2A contributes to the establishment and stabilization of differentiated epigenomes
Speaker : Marcus Buschbeck - IMPPC, Institute for Predictive and Personalized Medicine of Cancer, Spain
When : thursday 14 th February at 11 am
Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6)
Title : MacroH2A contributes to the establishment and stabilization of differentiated epigenomes
The most striking epigenetic alteration occuring on the level of the nucleosome is the complete exchange of a canonical histone H2A for a macroH2A variant. The expression level of macroH2A variants positively correlates with differentiation state of the cell. We have used loss of function approaches in zebrafish emryos, mouse embryonic stem cells and human tissue stem cells to demonstrate that macroH2A contributes to developmental and differentiation processes (1,2). Zebrafish embryos lacking macroH2A display several developmental defects. In mouse ES cells knockdown of macroH2A reduced their capacity to differentiate, best illustrated by a massive expansion of undifferentiated carcinoma tissue in derived teratomas. In the heterogenous culture of primary human keratinocytes macroH2A promoted differentiation and reduced the self-renewal capacity of the pluripotent compartment. Surprisingly in the light of macroH2A being intensively characterized as repressive, we found demonstrate that its pro-differentiation is at least partially dependent on a poorly recognized function favouring transcriptional activation. Novel insight from first high resolution ChIP-seq profiles will be discussed. Finally we will present unpublished results of an exciting researchline developed in collaboration with Dr. Bouvet and the ENS Lyon on the role of macroH2A in tissue regeneration.
Take home message : In line with the recent observation that macroH2A inhibits reprogramming (3) our results substantiate a role for macroH2A in the establishment and maintenance of differentiated epigenomes.
1. Creppe C, Janich P, Cantariño N, Noguera M, Valero V, Musulén E, Douet J, Posavec M, Martín-Caballero J, Sumoy L, Di Croce L, Benitah SA, Buschbeck M. MacroH2A1 regulates the balance between self-renewal and differentiation commitment in embryonic and adult stem cells. Mol Cell Biol (2012).
2. Buschbeck M, Uribesalgo I, Wibowo I, Rué P, Martin D, Gutierrez A, Morey L, Guigó R, López-Schier H, Di Croce L.The histone variant macroH2A is an epigenetic regulator of key developmental genes. Nat Struct Mol Biol (2009).
3. Histone variant macroH2A confers resistance to nuclear reprogramming. Pasque V, Gillich A, Garrett N, Gurdon JB. EMBO J. (2011)
Dans la même rubrique :
- Francesca Di Nunzio - HIV-1 docking at the nuclear pore, nuclear import and integration, concerted steps of HIV-1 infection
- Boudewijn van der Sanden - Microscopie intravitale pour les neurosciences, un monde interdisciplinaire pour explorer les cellules du système nerveux central dans l’animal entier
- Pierre-Alix dancer - Descriptif du Fluobeam et ses applications
- Vincent Calvez - Un modèle biomécanique pour l’interdigitation des sutures crâniennes.
- Daniel Riveline - Examples of phenomena in cell physics : cell motility and cell division
- Karine Monier / Xavier Gaume : Super-resolution microscopy
- Laurent Blanchoin - Directed actin self assembly and contractility
- Thomas Risler - Mechanics and stability of healthy and cancerous tissues
- Sylvain Gabriele - Adhesion, force and form : how cell mechanics control cellular functions ?
- Winfried Weissenhorn - Structural basis of ESCRT-driven release of enveloped viruses
- Jean-Baptiste Sibarita - Resolving molecular organization and dynamics of post-synaptic molecules using localization-based super-resolution microscopy
- Edouard Bertrand - RNA polymerase II : how cells assemble it and get genes transcribed
- Manouk Abkarian - Elastic prison break of malaria parasites : how to use the physics of the gift ribbon
- Christophe Godin - Modeling the mechanical regulation of morphogenesis in plants
- Franck Riquet - Optimized and reliable genetically encoded molecular tools for the visualization of cellular process dynamics.
- Massimo Vergassola - Finding the needle in a biological haystack
- Davide Normanno - Target search mechanisms of DNA-binding proteins : How to be at the right place at the right time ?
- Laurent Cognet - From long-term single molecule tracking to super-resolution imaging of complex molecular organizations in living cells.
- Christian Bergaud - Nanotechnology and Nanomaterials : promises for improved functional devices with applications in chemistry and biology.
- Bertrand Fourcade - From integrin activation to reaction-diffusion models : How podosomes organizations can be described as local self-sustained excitation waves of localized PdtIns(4,5)2 synthesis.
