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Accueil du site > Animations Scientifiques > Séminaires 2013 > Davide Normanno - Target search mechanisms of DNA-binding proteins : How to be at the right place at the right time ?
Davide Normanno - Target search mechanisms of DNA-binding proteins : How to be at the right place at the right time ?
Speaker : Dr. Davide Normanno, Ecole Normale Supérieure (ENS) and Institut Curie, Functional Imaging of Transcription Lab. Institut de Biologie de l’ENS (IBENS), Light-based Observation and Control of Cellular Organization Lab, Unité de Physico-Chimie Curie
E-mail : normanno@biologie.ens.fr Web : www.davidenormanno.com
When : thursday 4th july at 11 am
Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6)
Title :Target search mechanisms of DNA-binding proteins : How to be at the right place at the right time ?
Precise and timely expression of specific genes is of fundamental importance for the viability of any organism. Specific DNA-binding proteins (transcription factors, TFs) transiently associate to promoter sequences on the genome to activate or repress transcription of specific genes. The way TFs explore the nuclear space, find, and bind to their target sequences is the key to decipher transcriptional control in cells but is still poorly understood.
We exploit a live-cell model system to identify what factors primarily orchestrate TFs target search in human cells. The picture emerging from our experiments shows that specific DNA-binding proteins move in a very complex environment where they may remain locally confined. They interact transiently also with nonspecific DNA sequences, with broadly distributed residence times, and their target search mechanism is consistent with a non-compact exploration of the nuclear space with very inefficient binding at the target sites.
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