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Winfried Weissenhorn - Structural basis of ESCRT-driven release of enveloped viruses
Speaker :Winfried Weissenhorn, UVHCI, UMI 3265 University Joseph Fourier-EMBL-CNRS, Grenoble, France
When : thursday 25 th April at 11 am
Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6)
Title :Structural basis of ESCRT-driven release of enveloped viruses
Endosomal sorting complexes required for transport (ESCRTs) catalyze multivesicular body biogenesis, distinct steps during cell division and budding of some enveloped viruses including HIV-1. Although five complexes (ESCRT-0, -I, -II, -III, VPS4) constitute the endosomal ESCRT machinery, only ESCRT-III and VPS4 are recruited to all ESCRT-catalyzed processes thereby executing membrane fission. ESCRT-III proteins (CHMP1 to 6, IST1) are cytosolic and transiently recruited to cellular membranes upon activation. Membrane interaction in turn induces polymerization required for membrane remodeling steps. I will present data on the regulation of ESCRT-III by CC2D1A, Alix and AMSH and their effect on HIV-1 budding as well as structural and biochemical data on ESCRT-III polymer formation. I will present cryo electron microscopy data on ESCRT-III CHMP2A-CHMP3 polymers assembled in vitro and CHMP2B membrane tube polymers assembled in vivo. Based on these structures I will discuss a model for ESCRT-III catalyzed membrane fission.
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