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Internal Seminar — Gilles Charvin — Monitoring yeast replicative aging at the single-cell level
Speaker :
Gilles Charvin
When :
Wednesday 8 July at 11am
Where :
C023 (RDC LR6 côté CECAM)
Title :
Monitoring yeast replicative aging at the single-cell level
Abstract :
Aging is an intrinsic feature of living organisms which is partly genetically determined. Despite considerable efforts to identify the mechanisms that govern the transition to senescence, a precise and quantitative understanding at the cellular level is missing, mainly because it has been technically impossible to monitor biological processes over a long enough period of time.
We are currently developing a novel methodology to track in real-time the fate of a single yeast cell from birth to death using a microfluidics-based time-lapse microscope with extended capabilities. The principle of this device is to do long term imaging (typically 100 generations) in a controlled environment by limiting the growth of the colony using laser disruption of the progeny at the single cell level. By tracking the successive divisions of a unique cell, we will be able to observe and characterize the processes involved in replicative aging and we hope to decipher part of the mechanisms that lead to senescence.
After a long introduction about yeast replicative aging - which is intended to be widely accessible to non-specialists -, I will present the early stages of development of the imaging setup and show preliminary results. All LJC members are welcome to attend this talk, which will be more like a labmeeting rather than a formal lecture.
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