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Biophotonics in living cells
Orateur :
Laurent Héliot, Institut de Recherche Interdisciplinaire, Villeneuve d’Ascq
Salle :
C023 (RDC LR6 côté CECAM)
Quand :
Mercredi 18 Février à 11:00
Résumé :
The cell is a complex and dynamic 3D structure which functions are regulated by multi-protein complexe formation. Study of these complex assemblies is then a ubiquitous question in biology. Several techniques allow molecular interactions studies in cell. The FRET studies performed by fluorescence lifetime measurement is the best compromise between cell invasivity and result accuracy..
During this talk, I will present some optimizations we performed compared to classical lifetime measurement systems used in microscopy. First of all, we optimized instrumentation used in photon decay curves measurements with adapted excitation sources and detectors and several optimizations in data analysis. Even with such an acquisition system, several questions remain really complex to address using lifetime measurements alone because of fluorophores multi-parametric dependency towards molecular environment, experimental conditions and interpretation complexity. A solution to go further in the understanding of biological processes is to combine the acquisition and analysis of several characteristics of the emitted light. It is a multimodale fluorescence macroscopy. I will present the developpment and these applications in living cell and tissus.
KEY WORDS : Living cell, interactions, FRET, lifetime, FLIM, SLiM
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