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Accueil du site > Animations Scientifiques > Séminaires 2013 > Chistophe Zimmer - Microbiology through a computational lens : imaging & modeling approaches and applications to yeast nuclear architectureand HIV

Chistophe Zimmer - Microbiology through a computational lens : imaging & modeling approaches and applications to yeast nuclear architectureand HIV

Speaker : Christophe Zimmer, Unité Imagerie et Modélisation, Institut Pasteur

When : thursday 19th december at 11 am

Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6)

Title : Microbiology through a computational lens : imaging & modeling approaches and applications to yeast nuclear architectureand HIV

Our group develops computational and experimental imaging and modeling approaches for cell biology and microbiology.

In the first part of the talk, I will describe our work on the spatial organization of the yeast nucleus. Nuclear organization plays an important role in many functional processes, but has yet to be charted in detail, and the underlying principles remain poorly known. We will describe an imaging approach to map territories of chromosomal loci in the yeast nucleus and summarize insights into chromosome organization revealed by this method. We will then show that yeast nuclear organization can be largely understood and predicted quantitatively with a simple computational model based on polymer physics. Finally, we will present results on the implications of nuclear organization for DNA repairby homologous recombination.

In the second part of the talk, we will describe FlAsH-PALM, asuper-resolution imaging technique based on accurate localization of single FlAsH-labeled proteins. We will show how this method allowed non-invasive imaging of a delicate HIV protein at <30 nm resolution and enabled us to analyze the morphology of HIV in infected cells.This method should have broader applications in microbiology and opens a new door towards single nanometer resolution imaging.

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