Partenaires
Rechercher
Accueil du site > Animations Scientifiques > Séminaires 2007 > Molecular mechanisms of gene transcription and DNA repair in living cells
Molecular mechanisms of gene transcription and DNA repair in living cells
par Webmaster - 13 juin 2007
Orateur :
Adriaan Houtsmuller, Josephine Nefkens Institute, Erasmus University Rotterdam
Salle :
des Thèses
Sujet :
The molecular mechanisms underlying DNA-interacting processes inside the living cell nucleus such as gene transcription and DNA repair are poorly understood. To address this issue, we use GFP-tagging of transcription factors, and DNA-repair enzymes and study their function in living cells with confocal time-lapse imaging and quantitative fluorescence assays such as fluorescence recovery after photobleaching (FRAP) and fluorescence resonance energy transfer (FRET). In addition, for optimal interpretation of the complex and massive amount of experimental results we develop and apply computer modeling methodology to simulate both FRAP and FRET assays as well as the behavior of proteins in repair and transcription.
Dans la même rubrique :
- Discrete breathers in nonlinear network models of proteins
- Conservative behavior of the biological complex system [Telomeres-Telomerase-Prolifération]
- Linking molecular mechanisms to the organism’s physiology using transcriptomics
- Electron-donor modified nanoparticles for selected cell labeling and photodynamic therapy
- Soutenance d’HDR:Les modes normaux de basse fréquence des protéines
- Human monoclonal antibodies against viruses and cancer
- Vésicules biomimétiques à base de PIP2 pour étudier des interactions protéines membranes : application à une protéine de la famille des ERM (ezrine, radixine, moésine).
- Structural insights into base damage recognition and removal by the Fpg DNA glycosylase
- Transcription by yeast RNA polymerase III and Chromatin Remodeling
- Distinct roles of core histones, histone variants and linker histones in chromatin function
- DNA : More than just a ladder
- Chromatin dynamics in interphase
- On the role of the retroviral Gag protein and the RNA in virus assembly
- What determines the size of virus ?
- Cytoskeleton dynamics involved in cell morphology control and multicellular stability
- Models for the interaction between antibiotic peptides and lipid bilayers - Modèles pour l’interaction entre des peptides antibiotiques et des bicouches lipidiques
- Small Molecule Modulators of Chromatin Modifying Enzymes : To Probe Eukaryotic Transcription and To Target Diseases
- Exploration électrochimique de la dynamique de brins courts d’ADN ancrés sur une surface
- The nucleosome : A transparent, slippery, sticky and yet stable DNA-protein complex
- Histone H1 is a architectural molecule required for chromatin compaction, and is redundant for gene repression
