Solution and Solid-state NMR of Paramagnetic Molecules

13th-19th July, Florence, Italy
Organized as an EMBO Practical Course.
Please click on the link above to access the event website directly.

Followed by an Applied Training Course on

NMR Relaxation Theory

19th-20th July, Florence, Italy
Organised by Prof. Jozef Kowalewski from Stockholm University.

The SU training will take place at CERM on Saturday 19th July, and at the Hotel Villa Stanley on Sunday morning. Accommodations will be extended for the pNMR fellows for the 19th-20th July, and lunch will be provided on Saturday 19th July.

Location

The scientific campus (Polo Universitario) of the University of Florence.

Accommodation

Students and invited colleagues will stay in the same hotel, situated in the nearby scientific campus within 15 minutes walking distance. A transfer shuttle bus will be provided between the hotel and the scientific campus in the mornings and evenings.

All participants will be hosted on DUS room at the (Hotel Villa Stanley), an elegant and historical residence located in Sesto Fiorentino.

Content:

The EMBO Practical Course focuses on schemes for the acquisition of the NMR spectra of paramagnetic systems and on the analysis of the information that can be retrieved from the paramagnetic restraints. It will describe the main approaches used to characterize molecules and their complexes and on the characterization of the relaxation profiles of paramagnetic systems through field dependent measurements. We aim to cover hyperpolarization techniques, that are becoming increasingly important for improving the sensitivity of the NMR experiments, both in solution and in the solid state. In order not to limit the use of paramagnetism to metalloproteins, essential in this context is the use of rigidly binding lanthanide tags. Access will be provided to the advanced NMR instruments and software tools available at CERM.

The Stockholm University Training will consist of a course given by Prof. Jozef Kowalewski. It will expand and deepen some of the material he will present during the main Florence event mentioned above.

Programme

Day 1 – Sunday 13 July	14.30-18.30	Arrival and registration
	19.00	Welcome dinner
Day 2 – Monday 14 July	9.00-9.30	Introduction
	9.30-11.00	Plenary session: Claudio Luchinat – “Basics of paramagnetism”
	11.00-11.15	Coffee break
	11.15-12.45	Plenary session: Jozef Kowalewski – “Paramagnetic relaxation”
	12.45-14.15	Lunch with discussion tables
	14.15-16.15	Practical Session: Jozef Kowalewski – “Exercises on Paramagnetic relaxation”
	16.15-17.45	Poster session
	17.45-19.45	Practical session: Giacomo Parigi – “Acquisition of NMRD profiles”
	20.00	Dinner
Day 3 -Tuesday 15 July	9.00-11.00	Practical session:  “Acquisition of Paramagnetic NMR experiments”
	11.00-11.15	Coffee break
	11.15-12.45	Plenary session: Moreno Lelli – “Paramagnetic restraints for structure determination”
	12.45-14.15	Lunch with discussion tables
	14.15-16.15	Practical session: Jozef Kowalewski – “Analysis of NMRD profiles”
	16.15-16.45	Coffee break
	16.45-18.45	Practical session: Giacomo Parigi – “Analysis of NMRD profiles”
	19.30-22.30	Poster presentation’s @ Barbecue
Day 4 – Wednesday 16 July	9.00 -10.30	Plenary session: Marcellus Ubbink – “Paramagnetic tagging”
	10.30-10.45	Coffee break
	10.45-12.45	Practical session: Sandra Scanu – “Exercises on Paramagnetic tagging”
	12.45-14.30	Lunch with discussion tables
	14.30-16.30	Practical session: “Structure and dynamics through paramagnetic restraints”
	16.30-16.45	Coffee break
	16.45-18.45	Practical session: Moreno Lelli – “Structure and dynamics through paramagnetic restraints”
	19.30	Dinner
Day 5 -Thursday 17 July	9.00-10.30	Plenary session: Daniella Goldfarb – “EPR and DNP”
	10.30-10.45	Coffee break
	10.45-12.15	Plenary session: Björn Corzilius – “DNP and solid state NMR”
	12.15-12.45	Meet instructors and tutors
	12.45-14.15	Lunch with discussion tables
	14.15 – 16.15	Practical session: Sandra Scanu- “Protein-protein interactions through paramagnetic restraints”
	16.15-16.45	Coffee break
	16.45 -18.45	Practical session: Akiva Feintuch – “Exercises on DNP”
	18:45-19:45	Round Table Discussion
	20.00	Dinner
Day 6 – Friday 18 July	9.00-10.30	Plenary session: Tatyana Polenova – “Paramagnetic Solid State NMR”
	10.30-10.45	Coffee break
	10.45-12.15	Plenary session: Marina Bennati – “Hyperpolarization and liquid DNP”
	12.15-13.15	Lunch with discussion tables
	13.15-15.15	Practical session: “Acquisition of Solid state NMR spectra”
	15.15- 17.15	Practical session: Björn Corzilius – “Exercises on SS-NMR”
	17.15-17.30	Coffee break
	17.30- 19.30	Practical session: Tatyana Polenova – “Analysis of solid state NMR spectra”
	20.00	Course Dinner
Day 7 – Saturday 19 July : STOCKHOLM UNIVERSITY TRAINING	10.00-12.00	Jozef Kowalewski – Quantum mechanics for relaxation theory
	13.30-15.30	Jozef Kowalewski – Redfield relaxation theory
Day 8 – Sunday 20 July : STOCKHOLM UNIVERSITY TRAINING	10.00-12.00	Jozef Kowalewski – Time correlation functions and spectral densities

More details concerning the EMBO course program, registration and travel can be found on the event website here

More details about the Applied Training organised by Stockholm University will be advertised here soon.